| No. | Class | F.M. Whyte | Gauge | Railroad Line | Location | Status | Builder Info | Notes |
|---|
| | 0-4-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | 1831 | Named York, replica, from Chicago Museum of Science & Industry |
| | 0-4-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | | Rebuilt from #7 Grasshopper, Andrew Jackson to resemble the Atlantic. |
| | 0-4-0 | 4'-8½" | C&A | B&O Railroad Museum, Baltimore, MD | display | | Named Stourbridge Lion, replica. On loan from Smithsonian. |
| 8 | | 0-4-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | B&O (Mount Clare), 1836 | Named John Hancock |
| 1 | 35 ton | 0-4-0F | 4'-8½" | Potomac El Power | B&O Railroad Museum, Baltimore, MD | display | Heisler #43 | From Smithsonian Warehouse, Silver Hills |
| 4 (714) | | 0-4-0T | 4'-8½" | St. Elizabeth Hospital (USATC) | B&O Railroad Museum, Baltimore, MD | operational | H.K. Porter #8234, 1950 | |
| 57 | L | 0-8-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | New Castle Man., 1848 | Named Memnon. Sole survivor of this type. |
| | 2-2-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | operational | B&O (Mount Clare), 1927 | Named Tom Thumb, replica |
| 13 | | 2-2-2T | 4'-8½" | CVRR | B&O Railroad Museum, Baltimore, MD | display | Seth Wilmarth, 1851 | Named Pioneer. On loan from the Smithsonian Museum |
| 600 | K | 2-6-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | B&O (Mount Clare), 1875 | Named J.C. Davis. Damaged in 2003 roof collapse. |
| 1604 | H-8 | 2-6-6-6 | 4'-8½" | C&O | B&O Railroad Museum, Baltimore, MD | display | Lima #7824, 1941 | From Russell, KY the Roanoke, VA |
| 545 | E-8 | 2-8-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | B&O (Mount Clare), 1888 | Named A.J. Cromwell |
| 4500 | Q3 | 2-8-2 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | Baldwin #49153, 1918 | First USRA locomotive built |
| 2705 | K-4 | 2-8-4 | 4'-8½" | C&O | B&O Railroad Museum, Baltimore, MD | display | ALCO (Schenectady) #70854, 1943 | From Russell, KY |
| 13 | | 4-2-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | operational | B&O (Mount Clare), 1927 | Named Lafayette, replica |
| 25 | F | 4-4-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | Mason Machine Works #46, 1856 | Named William Mason, was oldest operational standard gauge steam locomotive on earth, featured in 'The Great Locomotive Chase' |
| 592 | P-6s / A-28 | 4-4-2C | 4'-8½" | CNJ | B&O Railroad Museum, Baltimore, MD | display | ALCO (Brooks) #25997, 1902 | |
| 377 (108) | F-11 | 4-6-0 | 4'-8½" | C&O (CR&M) | B&O Railroad Museum, Baltimore, MD | display | Baldwin #21329, 1902 | |
| 147 (117) | B | 4-6-0 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | B&O (Mount Clare), 1863 | Named Thatcher Perkins B |
| 1 | G7 | 4-6-0 | 4'-8½" | Clinchfield | B&O Railroad Museum, Baltimore, MD | display | CC&IC Logansport, 1882 | Last ran in 1979 |
| 305 (217) | A | 4-6-0C | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | B&O (Mount Clare), 1869 | Camel type locomotive |
| 5300 | P7 | 4-6-2 | 4'-8½" | B&O | B&O Railroad Museum, Baltimore, MD | display | Baldwin #59881, 1927 | Named President Washington |
| 490 | L1a | 4-6-4 | 4'-8½" | C&O | B&O Railroad Museum, Baltimore, MD | display | ALCO (Richmond) #66555, 1926 | Rebuilt from an F-19 Pacific boiler and streamlined |
| 1 (2101) | T-1 | 4-8-4 | 4'-8½" | Reading | B&O Railroad Museum, Baltimore, MD | display | Reading, 1945 | American Freedom Train Damaged in a roundhouse fire in Kentucky in 1979. |
| 1 | 80 ton | Shay3Tr | 4'-8½" | GC&E | B&O Railroad Museum, Baltimore, MD | display | Lima #1519, 1905 | |